rabbit adamts 1 antibody  (R&D Systems)

Journal: International Journal of Molecular Sciences

Article Title: Comparison of Steroidogenic and Ovulation-Inducing Effects of Orthosteric and Allosteric Agonists of Luteinizing Hormone/Chorionic Gonadotropin Receptor in Immature Female Rats

doi: 10.3390/ijms242316618

Figure Lengend Snippet: Immunohistochemical detection of metalloproteinase ADAMTS-1 on cross sections of the ovaries of immature female rats treated with “Follimag” and LH/hCG receptor agonists. The thickness of the sections was 10 µm. In all full-size photographs, the scale is 100 µm. Asterisks—preovulatory follicles, and CL—corpus luteum. The framed photographs show representative sections of the ovaries from the full-size photographs at higher magnification. Designations of animal groups: C1—control rats, corresponding to the start of animal treatment with LH/hCG receptor agonists (48 h after the beginning of the experiment); C2—control rats, corresponding to the end of the experiment; F48 and F72—rats treated with “Follimag” (15 IU/rat, s.c.) 48 or 72 h before being withdrawn from the experiment; FG4, FG8, FG16, and FG24—rats that were treated with “Follimag” and after 48 h were treated with hCG (15 IU/rat, s.c.), after which they were decapitated (after 4, 8, 16, and 24 h, respectively); FT4, FT8, FT16, and FT24—rats that were treated with “Follimag” and after 48 h were treated with TP4/2 (40 mg/kg, orally), after which they were decapitated (after 4, 8, 16, and 24 h, respectively).

Article Snippet: Sections were then incubated overnight at room temperature with rabbit anti-ADAMTS-1 primary antibodies (“Cloud-Clone Corp.”, Wuhan, China) diluted in blocking solution at a ratio of 1:25.

Techniques: Immunohistochemistry